| Rank | Time | Student | School | Date | |
| 1 | 2:00.24 | Victoria Kay Beeler | Park Hill South | 2/20/2016 | |
| 2 | 2:00.74 | Liz Smith | Parkway South | 2/17/2007 | |
| 3 | 2:01.06 | Liz Smith | Parkway South | 2/16/2007 | |
| 4 | 2:01.20 | Helena Dianne Tietjen | Pembroke Hill | 2/22/2025 | |
| 5 | 2:01.42 | Haley Spencer | Ft. Zumwalt West | 2/21/2009 | |
| 6 | 2:01.61 | Sydney Schoeck | Westminster Christian Academy | 2/17/2023 | |
| 7 | 2:01.88 | Kylee Anne Sullivan | Parkway South | 2/17/2023 | |
| 8 | 2:02.13 | Kylee Anne Sullivan | Parkway South | 2/19/2022 | |
| 9 | 2:02.18 | Liz Smith | Parkway South | 5/21/2005 | |
| 10 | 2:02.72 | Sydney Schoeck | Westminster Christian Academy | 2/18/2023 | |
| 11 | 2:02.79 | Hallie Burst Stupp | MICDS | 2/21/2009 | |
| 12 | 2:03.15 | Liz Smith | Parkway South | 5/20/2005 | |
| 13 | 2:03.43 | Nicole Williams | Rock Bridge | 2/17/2018 | Class 2 |
| 14 | 2:03.48 | Krystal M Caylor | Kickapoo | 2/18/2017 | |
| 15 | 2:03.65 | Victoria Kay Beeler | Park Hill South | 2/21/2015 | |
| 16 | 2:03.69 | Reagan Leigh Graybill | Staley | 2/21/2025 | |
| 17 | 2:06.00 | Reagan Leigh Graybill | Staley | 2/20/2025 | |
| 18 | 2:07.32 | Emma Brabham | Francis Howell | 2/17/2018 | Class 2 |
| 19 | 2:07.76 | Georgia Clark | Park Hill South | 2/17/2018 | Class 2 |
| 20 | 2:08.87 | Taylor Lynn Norwood | Poplar Bluff | 2/17/2018 | Class 2 |
| 21 | 2:09.05 | Lily Marie Harrell | Eureka | 2/17/2018 | Class 2 |
| 22 | 2:10.18 | Andrea Rogers | Lutheran St. Charles | 2/15/2018 | Class 1 |
| 23 | 2:10.23 | Annie Kreikemeier | St. Joseph's Academy | 2/15/2018 | Class 1 |
| 24 | 2:11.18 | Christina Jurotich | Visitation Academy | 2/16/2018 | Class 1 |
| 25 | 2:11.88 | Shelby Ripp | Parkway Central | 2/16/2018 | Class 1 |
| 26 | 2:12.27 | Madeleine Duncan | Glendale | 2/16/2018 | Class 1 |