| Rank | Time | Student | School | Date | |
| 1 | 1:00.43 | Haley Spencer | Ft. Zumwalt West | 2/21/2009 | |
| 2 | 1:01.24 | Liz Smith | Parkway South | 2/17/2007 | |
| 3 | 1:02.19 | Nicole Williams | Rock Bridge | 2/17/2018 | Class 2 |
| 4 | 1:02.37 | Caroline Foltz | Cor Jesu Academy | 2/17/2023 | |
| 5 | 1:02.55 | Haley Spencer | Ft. Zumwalt West | 5/20/2006 | |
| 6 | 1:02.79 | Nicole Williams | Rock Bridge | 2/18/2017 | |
| 7 | 1:02.86 | Caroline Foltz | Cor Jesu Academy | 2/16/2023 | |
| 8 | 1:02.87 | Reagan Leigh Graybill | Staley | 2/21/2025 | |
| 9 | 1:02.97 | Nicole Williams | Rock Bridge | 2/19/2016 | |
| 10 | 1:03.13 | Liz Smith | Parkway South | 5/21/2005 | |
| 11 | 1:03.15 | Caroline Foltz | Cor Jesu Academy | 2/18/2022 | |
| 12 | 1:03.60 | Haley Spencer | Ft. Zumwalt West | 5/19/2006 | |
| 13 | 1:03.71 | Jill Bastien | Parkway Central | 5/22/2004 | |
| 14 | 1:03.96 | Reagan Leigh Graybill | Staley | 2/20/2025 | |
| 15 | 1:03.97 | Liz Smith | Parkway South | 5/20/2005 | |
| 16 | 1:04.64 | Sarah Vogt | Hickman | 2/16/2018 | Class 2 |
| 17 | 1:04.81 | Ashlyn Canale | Visitation Academy | 2/22/2025 | |
| 18 | 1:05.14 | Abigail Levine | Parkway Central | 2/16/2018 | Class 1 |
| 19 | 1:05.45 | Ellie Zweifel | Rock Bridge | 2/17/2018 | Class 2 |
| 20 | 1:06.52 | Cabrini Josephvene Johnson | Logan-Rogersville | 2/16/2018 | Class 1 |
| 21 | 1:06.84 | Molly Harmon | Lee's Summit West | 2/17/2018 | Class 2 |
| 22 | 1:06.85 | Andrea Rogers | Lutheran St. Charles | 2/16/2018 | Class 1 |
| 23 | 1:07.40 | Annemarie Leigh Rehbein | Blue Springs | 2/17/2018 | Class 2 |
| 24 | 1:08.28 | Christina Jurotich | Visitation Academy | 2/15/2018 | Class 1 |
| 25 | 1:08.46 | Katherine Nelson | St. Dominic | 2/15/2018 | Class 1 |