| Rank | Time | Student | School | Date | |
| 1 | 4:50.71 | Macey Hansen | Battle | 2/16/2024 | |
| 2 | 4:51.43 | Macey Hansen | Battle | 2/17/2024 | |
| 3 | 4:51.71 | Alexis Lynn Cook | Parkway South | 2/21/2025 | |
| 4 | 4:51.82 | Alexis Lynn Cook | Parkway South | 2/20/2025 | |
| 5 | 4:54.12 | Avery Michelle TRUE | Liberty North | 2/17/2023 | |
| 6 | 4:54.33 | Macey Hansen | Battle | 2/17/2023 | |
| 7 | 4:54.44 | Paige Marcella Mitchell | Ladue Horton Watkins | 2/15/2019 | |
| 8 | 4:54.94 | Brittany Wen | Hickman | 2/20/2016 | |
| 9 | 4:55.53 | Heather Marie Lundstrom | Parkway North | 2/16/2013 | |
| 10 | 4:55.65 | Eleanor Grace Prewitt-Thomas | Blue Springs | 2/21/2009 | |
| 11 | 4:55.86 | Anna Moehn | Cor Jesu Academy | 2/18/2022 | |
| 12 | 4:56.33 | Anna Moehn | Cor Jesu Academy | 2/19/2022 | |
| 13 | 4:56.61 | Brigette M Selbert | Mehlville | 2/20/2009 | |
| 14 | 4:56.70 | Kelley Tackett | Rock Bridge | 2/16/2013 | |
| 15 | 4:56.90 | Brittany Wen | Hickman | 2/21/2015 | |
| 16 | 4:57.09 | Jamie Saxe | Lafayette (Wildwood) | 5/20/2000 | |
| 17 | 4:57.65 | Brittany Wen | Hickman | 2/18/2017 | |
| 18 | 4:58.48 | Michelle Tipton | Ft. Zumwalt West | 2/18/2011 | |
| 19 | 4:58.56 | Paige Marcella Mitchell | Ladue Horton Watkins | 2/17/2017 | |
| 20 | 4:58.71 | Anna-Grace G Guenther | St. Joseph's Academy | 2/22/2025 | |
| 21 | 4:59.80 | Grace Beahan | Hickman | 2/16/2018 | Class 2 |
| 22 | 5:05.37 | Madeleine Mather | Cor Jesu Academy | 2/16/2018 | Class 2 |
| 23 | 5:05.41 | Allison Weber | Marshall | 2/15/2018 | Class 1 |
| 24 | 5:06.14 | Caroline Liekweg | MICDS | 2/15/2018 | Class 1 |
| 25 | 5:06.65 | Joanna Dohrman | Francis Howell North | 2/17/2018 | Class 2 |
| 26 | 5:07.38 | Ansley Barnes | Rock Bridge | 2/16/2018 | Class 2 |
| 27 | 5:08.19 | Camille Lease | Hickman | 2/16/2018 | Class 2 |
| 28 | 5:08.45 | Cabrini Josephvene Johnson | Logan-Rogersville | 2/16/2018 | Class 1 |
| 29 | 5:13.20 | Maria Louise Newton | Parkway West | 2/15/2018 | Class 1 |
| 30 | 5:13.46 | Annie Kreikemeier | St. Joseph's Academy | 2/15/2018 | Class 1 |
| 31 | 5:54.94 | Brittany Wen | Hickman | 2/20/2016 | |