| Rank | Time | Student | School | Date | |
| 1 | 50.11 | Haiden Elizabeth Schoessel | Eureka | 2/17/2023 | |
| 2 | 50.19 | Taylor Wohrley | Timberland | 2/20/2009 | |
| 3 | 50.24 | Amber Renee Green | Eureka | 2/21/2009 | |
| 4 | 50.35 | Haiden Elizabeth Schoessel | Eureka | 2/16/2023 | |
| 5 | 50.36 | Anna Miller | St. Joseph's Academy | 2/18/2017 | |
| 6 | 50.48 | Avery Michelle TRUE | Liberty North | 2/17/2024 | |
| 7 | 50.51 | Kimberly A Steinhouse | Parkway West | 2/20/2009 | |
| 8 | 50.58 | Ashlyn Paige Moore | Kickapoo | 2/19/2022 | |
| 9 | 50.59 | Haiden Elizabeth Schoessel | Eureka | 2/17/2024 | |
| 10 | 50.63 | Avery Michelle TRUE | Liberty North | 2/16/2024 | |
| 11 | 50.69 | Liz Smith | Parkway South | 2/16/2007 | |
| 12 | 50.82 | Franceska Patricia Petrosino | Lafayette (Wildwood) | 2/17/2018 | Class 2 |
| 13 | 50.87 | Natalie S Dunaway | Park Hill South | 2/21/2025 | |
| 14 | 50.94 | Hannah Kinder | Central (Cape Girardeau) | 2/17/2007 | |
| 15 | 51.04 | Tarin Corwin | Blue Springs | 5/20/2006 | |
| 16 | 51.06 | Amber Renee Green | Eureka | 2/15/2008 | |
| 17 | 51.09 | Hannah Kinder | Central (Cape Girardeau) | 2/16/2007 | |
| 18 | 51.10 | Kirsten Mary-ellen Votava | Parkway South | 2/17/2018 | Class 2 |
| 19 | 51.20 | Franceska Patricia Petrosino | Lafayette (Wildwood) | 2/17/2018 | Class 2 |
| 20 | 51.29 | Kirsten Mary-ellen Votava | Parkway South | 2/17/2018 | Class 2 |
| 21 | 51.62 | Avery Allison Cunneen | St. Joseph's Academy | 2/22/2025 | |
| 22 | 51.73 | Ellie Wehrmann | Incarnate Word Academy | 2/16/2018 | Class 1 |
| 23 | 51.87 | Natalie S Dunaway | Park Hill South | 2/20/2025 | |
| 24 | 52.69 | Olivia Belle Henderson | Rock Bridge | 2/17/2018 | Class 2 |
| 25 | 53.07 | Ashley M Baldwin | Glendale | 2/16/2018 | Class 1 |
| 26 | 53.35 | Ashley M Baldwin | Glendale | 2/16/2018 | Class 1 |
| 27 | 53.49 | Andi Kreiling | Kearney | 2/16/2018 | Class 1 |
| 28 | 53.69 | Alexis Poe | Parkway Central | 2/16/2018 | Class 1 |