| Rank | Time | Student | School | Date | |
| 1 | 22.89 | Anna Miller | St. Joseph's Academy | 2/18/2017 | |
| 2 | 23.02 | Haiden Elizabeth Schoessel | Eureka | 2/17/2023 | |
| 2 | 23.02 | Haiden Elizabeth Schoessel | Eureka | 2/17/2024 | |
| 4 | 23.07 | Molly Moore | Summit Prep | 2/18/2017 | |
| 5 | 23.13 | Haiden Elizabeth Schoessel | Eureka | 2/17/2024 | |
| 6 | 23.19 | Molly Moore | Summit Prep | 2/15/2018 | Class 1 |
| 7 | 23.23 | Natalie S Dunaway | Park Hill South | 2/21/2025 | |
| 8 | 23.24 | Amber Renee Green | Eureka | 2/20/2009 | |
| 9 | 23.33 | Katherine L Gately | Parkway West | 2/18/2012 | |
| 9 | 23.33 | Reagan Cathcart | Francis Howell | 2/22/2020 | |
| 11 | 23.38 | Haiden Elizabeth Schoessel | Eureka | 2/19/2022 | |
| 12 | 23.39 | Tarin Corwin | Blue Springs | 5/20/2006 | |
| 12 | 23.39 | Hannah Kinder | Central (Cape Girardeau) | 2/16/2007 | |
| 14 | 23.43 | Kimberly A Steinhouse | Parkway West | 2/20/2009 | |
| 15 | 23.45 | Kirsten Mary-ellen Votava | Parkway South | 2/17/2018 | |
| 16 | 23.47 | Molly Moore | Summit Prep | 2/19/2016 | |
| 17 | 23.55 | Natalie S Dunaway | Park Hill South | 2/20/2025 | |
| 18 | 23.65 | Karsynne McAlister | Central (Cape Girardeau) | 2/22/2025 | |
| 19 | 23.67 | Reagan Cathcart | Francis Howell | 2/17/2018 | Class 2 |
| 20 | 23.68 | Autumn M. Looney | St. Charles | 2/15/2018 | Class 1 |
| 21 | 24.01 | Franceska Patricia Petrosino | Lafayette (Wildwood) | 2/17/2018 | Class 2 |
| 22 | 24.03 | Madelyn Hernandez | St. Joseph's Academy | 2/16/2018 | Class 1 |
| 22 | 24.03 | Bettie Logan | Rock Bridge | 2/16/2018 | Class 2 |
| 24 | 24.06 | Delaney Gayley Thomas | Lafayette (Wildwood) | 2/17/2018 | Class 2 |
| 25 | 24.11 | Madelyn Hernandez | St. Joseph's Academy | 2/16/2018 | Class 1 |
| 26 | 24.30 | Andi Kreiling | Kearney | 2/15/2018 | Class 1 |