| Rank | RB | Student | School | Opponent | Date | |
| 1 | 92 | | Higbee | Bunceton | 2/17/1983 | |
| 2 | 78 | | Higbee | Bevier | 1/23/2018 | |
| 3 | 77 | | North Callaway | Missouri School for the Deaf | 2/4/1980 | |
| 4 | 75 | | Twin Rivers | Greenville | 12/4/1989 | |
| 5 | 70 | | Grundy County | Tri-County | 2/15/2020 | |
| 6 | 69 | | Northwestern (Mendon) | Grundy County | 2/1/2007 | |
| 6 | 69 | | Higbee | | 2/4/2019 | |
| 6 | 69 | | Higbee | | 2/11/2020 | |
| 9 | 67 | | Kearney | Braymer | 11/13/1972 | |
| 9 | 67 | | Grandview (Hillsboro) | St. Louis Christian Acad. Sr. | 1/10/1989 | |
| 9 | 67 | | Santa Fe | Orrick | 2/12/1993 | |
| 9 | 67 | | Hillsboro | Ste. Genevieve | 12/17/2012 | |
| 9 | 67 | | Higbee | Marion County | 2/7/2018 | |
| 14 | 66 | | Scott County Central | Salisbury | 3/5/1982 | at UMC |
| 14 | 66 | | Scott County Central | Salisbury | 3/1/1982 | |
| 14 | 66 | | Scott County Central | Salisbury | 3/1/1982 | |
| 17 | 65 | | Lee's Summit North | Belton | 12/16/1999 | |
| 17 | 65 | | Cabool | Koshkonong | 12/11/2011 | |
| 17 | 65 | | Higbee | Meadville | 11/30/2017 | |
| 20 | 62 | | Scott County Central | Gainesville | 3/1/1982 | |
| 20 | 62 | | Scott County Central | Gainesville | 3/1/1982 | |
| 20 | 62 | | Meadville | Scott County Central | 3/1/1995 | |
| 20 | 62 | | Meadville | Scott County Central | 3/1/1996 | |
| 24 | 60 | | Central (New Madrid County) | Warrensburg | 3/1/1996 | |
| 24 | 60 | | Central (New Madrid County) | Warrensburg | 3/1/1996 | |
| 26 | 59 | | Northwest (Cedar Hill) | William Chrisman | 3/1/1993 | |
| 26 | 59 | | Northwest (Cedar Hill) | William Chrisman | 3/1/1993 | |
| 26 | 59 | | Lathrop | North Platte | 12/10/2020 | |
| 26 | 59 | | Lathrop | Van Horn | 1/13/2025 | |
| 30 | 57 | | Lathrop | Lexington | 11/23/2021 | |
| 31 | 55 | | Southern Boone | Community | 1/22/1999 | |
| 31 | 55 | | Higbee | | 2/18/2019 | |
| 33 | 54 | | Higbee | | 2/1/2019 | |
| 33 | 54 | | Higbee | | 1/29/2020 | |
| 33 | 54 | | Lathrop | Van Horn | 1/14/2021 | |
| 33 | 54 | | Lathrop | Penney | 2/11/2022 | |
| 37 | 53 | | Higbee | | 1/3/2020 | Vs Keytesville |
| 37 | 53 | | Lathrop | Gallatin | 11/20/2020 | |