| Rank | Student | School | Years | | AST | G | PerG |
| 1 | Alexis Uffmann | St. James | 2011-2014 | | 845 | 30 | 28.17 |
| 2 | Kelley Meier | Parkway North | 1985-1987 | | 942 | 83 | 11.35 |
| 3 | Kelly Walden | Rich Hill | 1985-1988 | | 959 | 117 | 8.20 |
| 4 | Kim Woodruff | St. James | 1994-1997 | | 937 | 115 | 8.15 |
| 5 | Becky Martin | Warren County | 1987-1989 | | 583 | 80 | 7.29 |
| 6 | Kenna Rae Walker | New Bloomfield | 1986-1989 | | 844 | 120 | 7.03 |
| 7 | Michelle McQueen | Lutheran South | 2004-2007 | | 794 | 116 | 6.84 |
| 8 | Jennifer Bowen | St. James | 2002-2005 | | 791 | 119 | 6.65 |
| 9 | Mikal Bencomo | Clopton | 2003-2006 | | 790 | 126 | 6.27 |
| 10 | Julie McQueen | Lutheran South | 2001-2004 | | 716 | 115 | 6.23 |
| 11 | Angela Scott | Thomas Jefferson Independent | 2002-2005 | | 619 | 101 | 6.13 |
| 12 | Heather R Harman | Walnut Grove | 2010-2013 | | 758 | 125 | 6.06 |
| 13 | Jayme Callahan | Rosary | 1995-1998 | | 663 | 115 | 5.77 |
| 14 | Jill Bybee | Skyline | 2001-2004 | | 689 | 121 | 5.69 |
| 15 | Brooklyn Duggan | Macks Creek | 2021-2024 | | 567 | 103 | 5.50 |
| 16 | Allie Heckemeyer | St. Elizabeth | 2010-2014 | | 587 | 109 | 5.39 |
| 17 | Madison Rae Ayers | South Iron | 2019-2022 | | 573 | 113 | 5.07 |
| 18 | Jade Berry | Delta | 2021-2024 | | 592 | 118 | 5.02 |
| 19 | Brooklyn Shaye Luna | Fair Grove | 2017-2020 | | 423 | 89 | 4.75 |
| 20 | Taylor Cook | Lesterville | 2008-2012 | | 451 | 95 | 4.75 |
| 21 | Kelsi McQuinn | Jefferson (Conception) | 2013-2016 | | 548 | 116 | 4.72 |
| 22 | Carlie Sanders | Hillsboro | 2014-2017 | | 451 | 103 | 4.38 |
| 23 | Sheridan Corum | Hermitage | 2014-2018 | | 476 | 111 | 4.29 |
| 24 | Payton Curley | Salem | 2013-2016 | | 407 | 103 | 3.95 |
| 25 | Lacy Stokes | Mt. Vernon | 2017-2020 | | 473 | 122 | 3.88 |
| 26 | Korrie Holcer | Meadville | 2020-2023 | | 413 | 107 | 3.86 |
| 27 | Paidyn Linville | West Nodaway | 2021-2024 | | 417 | 109 | 3.83 |
| 28 | Marley A Winkler | Northeast (Cairo) | 2012-2016 | | 403 | 113 | 3.57 |