| Rank | AST | Student | School | Opponent | Date | |
| 1 | 40 | | Macks Creek | Tuscumbia | 12/6/2019 | |
| 2 | 39 | | Meadow Heights | Marquand-Zion | 2/10/2006 | |
| 3 | 38 | | Pilot Grove | Missouri School for the Deaf | 1/31/1989 | |
| 4 | 37 | | Steelville | Kingston | 11/25/2003 | |
| 5 | 34 | | Twin Rivers | Naylor | 12/14/1978 | |
| 5 | 34 | | La Plata | Atlanta | 1/9/2007 | |
| 5 | 34 | | Grandview (Hillsboro) | St. Paul Lutheran (Farmington) | 12/29/2017 | |
| 5 | 34 | | Northeast Nodaway | North Harrison | 1/16/2021 | |
| 9 | 33 | | Niangua | Bradleyville | 2/6/2024 | |
| 10 | 32 | | Chadwick | New Covenant Academy | 2/16/2004 | |
| 10 | 32 | | Chadwick | New Covenant Academy | 3/2/2004 | at Monett |
| 12 | 30 | | St. Louis University | Metro Christian (St. Louis) | 1/14/1991 | |
| 12 | 30 | | Putnam County | Schuyler County | 3/3/1997 | |
| 12 | 30 | | Warrensburg | Oak Grove | 1/10/2003 | |
| 12 | 30 | | Jasper | Everton | 2/13/2004 | |
| 12 | 30 | | Meadville | Southwest (Livingston County) | 2/24/2025 | |
| 17 | 29 | | Macks Creek | | 1/18/2019 | vs. Pilot Grove |
| 18 | 28 | | Notre Dame (Cape Girardeau) | Monroe City | 3/1/1986 | |
| 18 | 28 | | Notre Dame (Cape Girardeau) | Monroe City | 1/1/1986 | |
| 18 | 28 | | Northeast Nodaway | King City | 12/11/2020 | |
| 21 | 27 | | Notre Dame (Cape Girardeau) | Hartville | 3/1/1987 | |
| 21 | 27 | | Notre Dame (Cape Girardeau) | Hartville | 1/1/1987 | |
| 23 | 26 | | Bishop LeBlond | St. James | 3/1/1975 | |
| 23 | 26 | | Bishop LeBlond | St. James | 1/1/1975 | |
| 23 | 26 | | Northeast Nodaway | Lockwood | 3/1/1986 | |
| 23 | 26 | | Northeast Nodaway | Lockwood | 1/1/1986 | |
| 23 | 26 | | Macks Creek | | 11/20/2018 | |
| 23 | 26 | | Northeast Nodaway | DeKalb | 12/22/2020 | |
| 29 | 24 | | Webster Groves | William Chrisman | 3/1/1969 | |
| 29 | 24 | | Webster Groves | William Chrisman | 1/1/1969 | |
| 29 | 24 | | Greenfield | Advance | 1/1/1974 | |
| 29 | 24 | | Greenfield | Advance | 3/1/1974 | |
| 29 | 24 | | Scott County Central | New Bloomfield | 3/1/1991 | |
| 29 | 24 | | Scott County Central | New Bloomfield | 1/1/1991 | |
| 29 | 24 | | Hartville | Portageville | 3/1/1998 | |
| 29 | 24 | | Hartville | Portageville | 1/1/1998 | |
| 29 | 24 | | St. Pius X (Festus) | | 2/4/2020 | |
| 29 | 24 | | Northeast Nodaway | North Harrison | 2/8/2022 | |