| Rank | RB | Student | School | Opponent | Date | |
| 1 | 47 | Jeff Cox | Marquand-Zion | Greenville | 2/4/1976 | at Zalma |
| 2 | 42 | Leo Goolsby | Zalma | Marquand | 12/6/1963 | |
| 2 | 42 | Jim Lewis | Glasgow | McCooey (Hannibal) | 3/4/1964 | |
| 4 | 41 | Jim Lewis | Glasgow | Bunceton | 1/22/1964 | |
| 4 | 41 | Jim Lewis | Glasgow | Harrisburg | 2/29/1964 | |
| 6 | 39 | Leo Goolsby | Zalma | Leopold | 11/22/1963 | |
| 6 | 39 | Keith Morrissey | Gallatin | Bethany | 12/10/1971 | |
| 6 | 39 | Keith Morrissey | Gallatin | Grant City | 1/24/1972 | |
| 9 | 38 | Larry Britt | Callao High School | Atlanta | 10/22/1970 | |
| 10 | 37 | Carl Brown | Westran | New Franklin | 12/22/1970 | |
| 10 | 37 | Scott Wolfe | Kemper | Higbee | 2/16/1973 | |
| 12 | 35 | Otto L. Porter | Scott County Central | Pilot Grove | 3/1/2010 | |
| 13 | 30 | Caleb Walker | Eugene | Versailles | 11/23/2021 | |
| 14 | 29 | Jaylen Thomas | Metro | Gateway Science Charter | 1/12/2018 | |
| 14 | 29 | Damon Whittle | Tuscumbia | Missouri Military Academy | 12/5/2025 | |
| 16 | 28 | Paul Miller | Blair Oaks | Butler | 3/8/2000 | |
| 16 | 28 | Lucas Pisoni | Fox | Affton | 2/3/2020 | |
| 18 | 26 | Hunter Lane | Macks Creek | | 11/26/2018 | |
| 18 | 26 | Aminu Mohammed | Greenwood | Marion C. Early | 2/18/2019 | |
| 20 | 25 | Bill Flamank | Raytown South | Hickman | 12/22/1969 | |
| 20 | 25 | Otto Porter Sr. | Scott County Central | Glasgow | 3/1/1976 | |
| 20 | 25 | Aminu Mohammed | Greenwood | Skyline | 12/28/2018 | |
| 20 | 25 | Caleb Maassen | Linn | Linn | 2/5/2021 | |
| 24 | 24 | Bill Flamank | Raytown South | Nevada | 3/5/1970 | |
| 24 | 24 | Edgar Wilson | Eskridge | Mansfield | 3/1/1985 | |
| 24 | 24 | Aminu Mohammed | Greenwood | Billings | 12/11/2018 | |
| 24 | 24 | Aminu Mohammed | Greenwood | Fordland | 1/25/2019 | |
| 28 | 23 | Lamont Frazier | Charleston | Marshfield | 3/1/1989 | |
| 28 | 23 | Aminu Mohammed | Greenwood | Glendale | 12/29/2018 | |
| 28 | 23 | Aminu Mohammed | Greenwood | Plattsburg | 3/8/2019 | |
| 28 | 23 | Kyle Renkoski | Pierce City | Verona | 12/4/2020 | |
| 32 | 22 | K. Ruble | South Iron | Jameson | 3/1/1963 | |
| 32 | 22 | Ray Lavender | Lindbergh | Augustinian | 3/1/1966 | |
| 32 | 22 | Otto Porter Sr. | Scott County Central | Drexel | 3/1/1976 | |
| 32 | 22 | James Fleener | Polo | Polo | 1/20/2017 | |
| 32 | 22 | Aminu Mohammed | Greenwood | Billings | 12/7/2019 | |
| 32 | 22 | Aminu Mohammed | Greenwood | Crane | 1/29/2020 | |
| 32 | 22 | Aminu Mohammed | Greenwood | Christ the King | 1/18/2020 | |
| 32 | 22 | Brian Cole Peterson | Grundy County | Winston | 2/2/2023 | |
| 32 | 22 | Devin Harrington | Thayer | Lutheran North | 1/1/2023 | |
| 41 | 21 | Bill Flamank | Raytown South | Oak Park | 1/16/1970 | |
| 41 | 21 | | Raytown South | Truman | 2/13/1970 | |
| 41 | 21 | Bill Flamank | Raytown South | Hannibal | 3/11/1970 | |
| 41 | 21 | Anthony Frazier | Greenwood | Marion C. Early | 3/1/1982 | |
| 41 | 21 | Will Bogan | Bell City | La Plata | 3/1/2007 | |
| 41 | 21 | Clayson D Patterson | Chilhowee | | 2/22/2018 | |
| 41 | 21 | Grady Todd | Plato | | 11/26/2019 | |
| 41 | 21 | Parker Kaullen | Lexington | Carrollton | 12/1/2022 | |
| 41 | 21 | Stephen Okoro | Principia | Salisbury | 1/1/2023 | |
| 41 | 21 | Jack Stephens | Galena | Galena | 12/17/2024 | |
| 41 | 21 | Jack Stephens | Galena | Purdy | 2/17/2025 | |
| 52 | 20 | Damon Whittle | Tuscumbia | Iberia | 11/26/2024 | |
| 53 | 19 | | Raytown South | Lindbergh | 3/13/1970 | State Semifinal |
| 54 | 17 | Jamarious White | Senath-Hornersville | Holcomb | 12/9/2025 | |
| 55 | 16 | Bill Flamank | Raytown South | Rockhurst | 3/14/1970 | |