| Rank | AST | Student | School | Opponent | Date | |
| 1 | 30 | Jacob Asher | Bell City | Zalma | 1/6/2026 | |
| 2 | 25 | Charles Robbins | Clearwater | Marquand-Zion | 12/13/2022 | |
| 3 | 22 | Jon Sundvold | Blue Springs | Ruskin | 1/26/1979 | |
| 4 | 20 | Stan Jenkins | Jennings | Lutheran North | 3/2/1979 | |
| 4 | 20 | Rod Gorman | Licking | Cabool | 1/21/1983 | |
| 4 | 20 | Dave Boyer | DeSoto | Windsor (Imperial) | 2/9/1988 | |
| 4 | 20 | Demond McClelland | Riverview Gardens | Normandy | 2/12/1993 | |
| 4 | 20 | Nathan Frazier | Versailles | Cole Camp | 2/22/2005 | |
| 4 | 20 | Aaron Buford | Scotland County | Milan | 2/26/2015 | |
| 10 | 19 | Douglas Mattson | Stanberry | Pattonsburg | 2/27/1986 | |
| 10 | 19 | Ronie Ressel | Notre Dame (Cape Girardeau) | Monroe City | 3/14/1986 | At. MSU |
| 10 | 19 | Ronnie Ressel | Notre Dame (Cape Girardeau) | Monroe City | 3/1/1986 | |
| 10 | 19 | Ryan Robertson | St. Charles West | Wentzville | 1/13/1995 | |
| 10 | 19 | Tim Lowery | Scott City | Hayti | 1/30/1998 | |
| 15 | 17 | Ronnie Ressel | Notre Dame (Cape Girardeau) | Hartville | 3/1/1987 | |
| 16 | 16 | Brandon Blankenship | Risco | North Pemiscot | 12/6/1994 | |
| 16 | 16 | Ethan Scheetz | Niangua | Bradleyville | 2/6/2024 | |
| 18 | 15 | Travis Smith | Diamond | Monroe City | 3/1/1995 | |
| 18 | 15 | Cameron Cooper | Concordia | | 2/2/2017 | vs. Leeton |
| 18 | 15 | Reese Daniel Murdock | Versailles | Fatima | 1/28/2023 | |
| 21 | 14 | Ronnie Ressel | Notre Dame (Cape Girardeau) | Lathrop | 3/1/1987 | |
| 21 | 14 | Oscar Cortez | Macks Creek | | 12/28/2018 | vs. Montrose |
| 21 | 14 | Aaron Peavler | Green City | Gilman City | 1/13/2022 | |
| 24 | 13 | Danny Dunkman | Webster Groves | William Chrisman | 3/1/1969 | |
| 24 | 13 | Bobby Hatchett | Scott County Central | Newtown-Harris | 3/1/2009 | |
| 24 | 13 | Cameron Cooper | Concordia | | 2/10/2017 | vs. Santa Fe |
| 24 | 13 | Oscar Cortez | Macks Creek | | 12/11/2018 | vs. Dadeville |
| 24 | 13 | Cameron Cooper | Concordia | | 1/30/2018 | |
| 24 | 13 | Cameron Cooper | Concordia | | 1/9/2018 | vs. Crest Ridge |
| 30 | 12 | Michael Morris | Charleston | Warrensburg | 3/1/1983 | |
| 30 | 12 | Kyle Wolfe | New Haven | Bishop Hogan High | 3/1/1997 | |