| Rank | AST | Student | School | Opponent | Date | |
| 1 | 7 | Sidney Herner | Bishop LeBlond | Benton | 3/20/2017 | |
| 2 | 6 | Stephanie Fromso | Grandview | Lincoln College Prep | 4/16/1991 | |
| 2 | 6 | Jackie Entsminger | Rolla | Fulton | 5/8/2000 | |
| 2 | 6 | Emily A Jordan | Republic | Monett | 4/11/2015 | |
| 2 | 6 | Madelin McConnell | Summit Christian Academy | | 4/9/2019 | |
| 6 | 5 | Natasha Lord | Waynesville | Hillcrest | 4/5/2005 | |
| 6 | 5 | Kaylee Smith | Platte County | Maryville | 5/2/2007 | |
| 6 | 5 | Nicole Jolene Lucas | Truman | William Chrisman | 5/8/2009 | |
| 6 | 5 | Mercedes Estes | Central (St. Joseph) | Benton | 3/28/2011 | |
| 6 | 5 | Emily Boberg | Crocker | | 4/23/2019 | |
| 11 | 4 | Leslie Slais | Waynesville | Northwest (Cedar Hill) | 4/20/1996 | |
| 11 | 4 | Jessica Lenzen | Northwest (Cedar Hill) | Pacific | 5/14/1999 | |
| 11 | 4 | Angela Long | Lee's Summit | William Chrisman | 5/3/1999 | |
| 11 | 4 | Lisa Case | Smith-Cotton | Excelsior Springs | 5/16/1999 | |
| 11 | 4 | Kaylee Smith | Platte County | Excelsior Springs | 4/9/2007 | |
| 11 | 4 | Tara Rose | Platte County | Savannah | 4/27/2007 | |
| 11 | 4 | Sarah Nicole Franklin | Truman | Barstow | 4/3/2008 | |
| 11 | 4 | Kennedy Seifert | Moberly | Fulton | 4/12/2009 | |
| 11 | 4 | Haley Kirsten Baker | Camdenton | St. Paul (Stover) Jr. | 3/29/2010 | |
| 11 | 4 | Cassandra Elizabeth Hale | Camdenton | Central (Springfield) | 5/12/2010 | |
| 11 | 4 | Shelly Renegar | Liberty (Mountain View) | Pleasant Hope | 5/14/2010 | |
| 11 | 4 | Natalie Henry | Liberty (Mountain View) | Hillcrest | 4/22/2013 | |
| 11 | 4 | Bailey Wagner | Liberty (Mountain View) | St. James | 3/27/2014 | |
| 11 | 4 | Ashley Marie Talley | Neosho | | 4/30/2016 | |
| 11 | 4 | Ashley Marie Talley | Neosho | | 5/2/2016 | |
| 11 | 4 | Lynsey Powers | Kelly | | 5/2/2016 | |
| 11 | 4 | Jersee R Wren | Southern Boone | Marshall | 5/1/2021 | |
| 11 | 4 | Grace M Dunn | Southern Boone | Boonville | 3/29/2022 | |
| 11 | 4 | Allison Jorja Estanich | Park Hill | Blue Springs | 4/12/2022 | |
| 11 | 4 | Caroline Rose Mills-Hosmer | Central (Springfield) | Parkview | 3/21/2023 | |
| 11 | 4 | Caroline Rose Mills-Hosmer | Central (Springfield) | Lebanon | 4/16/2024 | |
| 11 | 4 | Laramie Sage Caswell Hill | Central (Springfield) | Parkview | 3/19/2024 | |
| 11 | 4 | Ava Leininger | Southern Boone | Blair Oaks | 4/7/2025 | |
| 11 | 4 | Claire Monell Brightwell | Francis Howell North | Francis Howell North | 4/15/2025 | |
| 35 | 3 | Brianna Mueller | Saxony Lutheran | Father Tolton Regional Catholic | 6/6/2015 | |
| 35 | 3 | Macy Laine Trujillo | St. Teresa's Academy | Francis Howell Central | 6/6/2015 | |
| 35 | 3 | Lauren Christine Seppi | Nerinx Hall | Liberty North | 6/3/2023 | |