| Rank | Time | Student | School | Opponent | Date | |
| 1 | 0:05 | Grace Ana Larson | Osage | Fulton | 4/22/2024 | |
| 2 | 0:09 | Isabelle Shackelford | Lee's Summit | Blue Springs | 4/16/2018 | |
| 3 | 0:10 | Anne Felts | Rolla | West Plains | 4/3/2001 | |
| 4 | 0:11 | Bailey Welch | Hannibal | West Plains | 4/23/2016 | |
| 4 | 0:11 | Amanda Mantel | Liberty (Mountain View) | Crocker | 3/17/2018 | |
| 4 | 0:11 | Malia Belle Stolte | Hannibal | Moberly | 3/28/2024 | |
| 7 | 0:12 | Raylynn Charlton | Hannibal | Elsberry | 5/5/2014 | |
| 7 | 0:12 | Britny Baker | Lebanon | Crocker | 4/12/2018 | |
| 9 | 0:13 | K.C. Williams | Rolla | Waynesville | 5/3/1998 | |
| 9 | 0:13 | Shelbey A Johnson | Poplar Bluff | Liberty (Mountain View) | 5/9/2017 | |
| 11 | 0:14 | Jessica Reeter | Chillicothe | Cameron | 5/2/2023 | |
| 12 | 0:15 | Kristi Elizabeth Blau | Truman | Fort Osage | 5/19/2010 | |
| 12 | 0:15 | Aspen Patricia Hawkins | Central (Springfield) | Monett | 3/29/2014 | |
| 14 | 0:16 | Faith Coates | Rolla | Rolla | 4/20/2021 | |
| 15 | 0:17 | Bri Johnson | Hannibal | Canton | 3/26/2014 | |
| 15 | 0:17 | Baylei Palmer | Lebanon | Osage | 4/10/2018 | |
| 17 | 0:18 | Harlie Jones | Chillicothe | Maryville | 4/17/2018 | |
| 18 | 0:19 | Lydia Meusch | Rolla | Rolla | 4/13/2021 | |
| 18 | 0:19 | Madison Elizabeth Morgan | Ozark | Capital City | 5/6/2022 | |
| 20 | 0:21 | Whitney Nichole Snow | Camdenton | Joplin | 5/14/2014 | |
| 20 | 0:21 | Kenna Bret Hallquist | Mid-Buchanan | East (Kansas City) | 3/30/2021 | |
| 22 | 0:22 | Sommer Herner | Bishop LeBlond | Cameron | 4/19/2018 | |
| 23 | 0:23 | Julia Richardson | Southern Boone | St. Charles West | 4/14/2023 | |
| 24 | 0:24 | Grace Ana Larson | Osage | Fulton | 5/4/2022 | |
| 25 | 0:25 | Grace White | Elsberry | Blair Oaks | 6/7/2025 | Class 1 |
| 26 | 0:26 | Jersee R Wren | Southern Boone | Winfield | 5/3/2021 | |
| 27 | 0:32 | Olivia Grace Brinkman | Central (Springfield) | New Covenant Academy | 5/6/2021 | |
| 27 | 0:32 | Alyssa Rene'e Newberry | Osage | Mexico | 4/25/2023 | |
| 29 | 0:34 | Journey Blaze Caswell Hill | Central (Springfield) | Lebanon | 4/22/2025 | |
| 30 | 0:35 | Holland Hawkins | Central (Springfield) | West Plains | 4/17/2021 | |
| 30 | 0:35 | Journey Blaze Caswell Hill | Central (Springfield) | Carl Junction | 5/19/2025 | |
| 32 | 0:36 | Isabelle Marie Milligan | Osage | Battle | 4/16/2024 | |
| 33 | 0:38 | Grace Ana Larson | Osage | Richland | 4/17/2023 | |
| 34 | 0:42 | Holland Hawkins | Central (Springfield) | | 4/25/2019 | vs Lebanon |
| 35 | 11:49 | Amanda Mantel | Liberty (Mountain View) | Crocker | 3/17/2018 | |