| Rank | AST | Student | School | Opponent | Date | |
| 1 | 6 | Zachary Cobb | Northwest (Cedar Hill) | St. Charles | 8/30/2004 | |
| 1 | 6 | Cody Brill | Harrisonville | Oak Grove | 9/13/2011 | |
| 1 | 6 | Mark Moore | Hillsboro | | 9/18/2018 | vs St. Clair |
| 4 | 5 | Jay Shelby | Moberly | St. Thomas Seminary | 9/23/1995 | At Fulton |
| 4 | 5 | Matt Maher | Kickapoo | Carthage | 11/4/1998 | |
| 4 | 5 | Bryan Wear | Pembroke Hill | Lincoln College Prep | 11/5/2002 | |
| 4 | 5 | Jon Rogers | Maryville | Cameron | 9/17/2007 | |
| 4 | 5 | Gino Bono | Northwest (Cedar Hill) | Festus | 10/24/2012 | |
| 4 | 5 | Rory McKeown | Mexico | Fulton | 10/19/2015 | |
| 4 | 5 | Riley Moore | Smith-Cotton | Center | 8/24/2017 | |
| 4 | 5 | Grant Hoehne | Southern Boone | Missouri Military Academy | 9/9/2020 | |
| 4 | 5 | Ethan Larson | Laquey | Iberia | 9/1/2022 | |
| 4 | 5 | Gauge Fleschute | Laquey | Parkview | 9/2/2022 | |
| 4 | 5 | Ethan Larson | Laquey | Iberia | 10/1/2022 | |
| 4 | 5 | Tristen Carriger | Laquey | Iberia | 10/1/2022 | |
| 4 | 5 | Emanuele Riva | Stover | La Monte | 10/10/2023 | |
| 17 | 4 | Steve Kickham | Rosary | Lutheran South | 11/1/1985 | |
| 17 | 4 | Geoff Rigsby | Waynesville | Warrenton | 9/17/1994 | at Rolla |
| 17 | 4 | Nathan Allen | Hazelwood East | McCluer | 11/2/1996 | |
| 17 | 4 | Chris Fox | Grandview | Excelsior Springs | 11/26/1997 | |
| 17 | 4 | Darren Stewart | Park Hill South | Center | 10/29/1998 | at Park College |
| 17 | 4 | Shane Skinner | Waynesville | Kennett | 9/29/2001 | at West Plains |
| 17 | 4 | Nick Golden | Grandview | Ruskin | 10/26/2001 | |
| 17 | 4 | Brandon LaMar | Waynesville | Aurora | 9/23/2004 | at Bolivar |
| 17 | 4 | Jarrid Hamilton | Northwest (Cedar Hill) | DeSoto | 10/2/2013 | |
| 17 | 4 | Jarrid Hamilton | Northwest (Cedar Hill) | Festus | 10/28/2013 | |
| 17 | 4 | Joe Noah | Northwest (Cedar Hill) | Parkview | 9/17/2016 | |
| 17 | 4 | Cory Allensworth | Hannibal | | 8/25/2017 | Elsberry |
| 17 | 4 | Rylen Ballard | Logan-Rogersville | Hillcrest | 9/2/2021 | |
| 17 | 4 | Braidon Rowden | Iberia | Richland | 11/1/2021 | |
| 17 | 4 | Braidon Rowden | Iberia | La Monte | 9/7/2021 | |
| 17 | 4 | Ethan Larson | Laquey | Aurora | 9/26/2022 | |
| 17 | 4 | Bodie Rollins | Hannibal | Warrenton | 10/24/2024 | |
| 34 | 3 | Tristen Carriger | Laquey | Iberia | 8/31/2023 | |
| 34 | 3 | Ethan Larson | Laquey | Iberia | 8/31/2023 | |
| 34 | 3 | Ethan Larson | Laquey | La Monte | 10/13/2023 | |
| 34 | 3 | Bodie Rollins | Hannibal | Mexico | 10/17/2024 | |
| 34 | 3 | Bodie Rollins | Hannibal | Fulton | 9/26/2024 | |