| Rank | Time | Student | School | Opponent | Date | |
| 1 | 0:06 | Casey St. Clair | Hannibal | Canton | 8/8/2012 | |
| 1 | 0:06 | Tyler Gillespie | Lone Jack | Warrensburg | 9/8/2016 | |
| 3 | 0:07 | Jim Roth | Christian Brothers College | Bishop DuBourg | 12/1/1973 | |
| 4 | 0:08 | Isaac Pearson | Maplewood-Richmond Hts. | Valley Park | 9/8/2017 | |
| 4 | 0:08 | Parker Bata | Lincoln College Prep | Bayless | 11/3/2017 | State Semifinal |
| 4 | 0:08 | Brycen A Chura | Lone Jack | La Monte | 10/19/2023 | |
| 7 | 0:09 | Anthony Dominic Alberti | Osage | Camdenton | 10/22/2020 | |
| 7 | 0:09 | Jack Edward Brinkman | Central (Springfield) | Monett | 9/25/2023 | |
| 7 | 0:09 | Dalton Anderson | New Covenant Academy | Monett | 10/30/2023 | |
| 10 | 0:10 | Isaac Pearson | Maplewood-Richmond Hts. | Principia | 9/14/2017 | |
| 10 | 0:10 | Austin Roy Hoffman | Marshfield | Aurora | 9/13/2022 | |
| 12 | 0:11 | Ethan Roth | Moberly | Hannibal | 9/14/2011 | |
| 12 | 0:11 | Dixon Yosimar Alvarez, I | Center | Warrensburg | 10/23/2025 | |
| 14 | 0:13 | Zachary Richards | Lebanon | Osage | 9/20/2018 | |
| 14 | 0:13 | Rex Taylor | Center | Oak Grove | 9/23/2025 | |
| 16 | 0:14 | Jacob Elliott | Pleasant Hill | Odessa | 10/27/2020 | |
| 16 | 0:14 | Haden Maddox Gohdes | Lone Jack | Summit Christian Academy | 9/30/2025 | |
| 18 | 0:17 | William Harrison Faulconer | Osage | Capital City | 9/13/2022 | |
| 19 | 0:21 | Caleb Lombardino | Bishop LeBlond | Frontier STEM | 10/16/2017 | |
| 20 | 0:22 | Drew Dial | Southern Boone | Mexico | 9/27/2022 | |
| 21 | 0:25 | Erik Petersen | Lutheran North | Priory | 9/22/2003 | |
| 21 | 0:25 | Miles Butler | Southern Boone | Fulton | 10/16/2025 | |
| 23 | 0:27 | Grant Hoehne | Southern Boone | Canton | 11/2/2020 | |
| 24 | 0:30 | Mike McNicholas | Ritenour | Mehlville | 9/30/1978 | (at UMSL) |
| 24 | 0:30 | Sean Snedeker | Ritenour | Clayton | 10/29/1998 | |
| 26 | 0:33 | Aaron White | Hannibal | Boonville | 9/6/2012 | |
| 27 | 0:34 | Max David Robertson | Central (Springfield) | Fair Grove | 9/12/2024 | |
| 28 | 0:37 | Carson Whittle | Iberia | Maranatha Baptist Academy | 9/17/2020 | |
| 29 | 0:40 | Logan James Canada | Central (Springfield) | Green Ridge | 10/4/2025 | |
| 30 | 16:30 | Austin Roy Hoffman | Marshfield | Aurora | 9/13/2022 | |