| Rank | Time | Student | School | Date | |
| 1 | 48.12 | Cooper Jeffrey Scharff | SLUH | 11/13/2021 | Class 2 |
| 2 | 48.28 | Andrew Jacob Baklanov | Rockhurst | 11/15/2024 | |
| 3 | 48.37 | Caden Scott Francois | Rockhurst | 11/11/2022 | |
| 4 | 48.92 | Jonathan Lee Naber | MICDS | 11/13/2021 | |
| 5 | 48.96 | Andrew Jacob Baklanov | Rockhurst | 11/14/2024 | |
| 6 | 48.98 | Robert Hill | Oak Park | 11/15/2019 | Class 1 |
| 7 | 48.99 | Nicholas Anatoli Alexander | Vianney | 11/7/2015 | |
| 8 | 49.15 | Jonathan Lee Naber | MICDS | 11/12/2021 | |
| 9 | 49.16 | Jacob Bening | Central (Cape Girardeau) | 11/15/2019 | |
| 10 | 49.24 | Caden Scott Francois | Rockhurst | 11/10/2022 | |
| 10 | 49.24 | Austin ManHei Chu | Clayton | 11/16/2024 | |
| 12 | 49.28 | Robert Hill | Oak Park | 11/14/2019 | |
| 13 | 49.37 | Jonathan Lee Naber | MICDS | 11/14/2020 | |
| 14 | 49.51 | Scott Jostes | Chaminade | 11/11/2006 | |
| 15 | 49.54 | Graham Henderson | Liberty | 11/15/2024 | |
| 16 | 49.67 | Jack Wever | Kirkwood | 11/5/2016 | |
| 16 | 49.67 | Andrew Riley Hopkins | SLUH | 11/15/2024 | |
| 18 | 49.72 | Nicholas Anatoli Alexander | Vianney | 11/8/2014 | |
| 18 | 49.72 | Jack Thomas Wever | Kirkwood | 11/4/2017 | Class 2 |
| 20 | 49.75 | Andrew Riley Hopkins | SLUH | 11/14/2024 | |
| 21 | 49.76 | Matthew Charles Maurer | Kickapoo | 11/15/2008 | |
| 22 | 49.98 | Trevor (t.j.) James Halliburton | Chaminade | 11/5/2016 | |
| 23 | 50.09 | Austin ManHei Chu | Clayton | 11/15/2024 | |
| 24 | 50.18 | Scott Jostes | Chaminade | 2/11/2006 | |
| 24 | 50.18 | Thomas J Auner | Greenwood | 11/10/2007 | |
| 26 | 50.23 | Kevin Velleca | Ft. Zumwalt South | 2/10/2001 | |
| 27 | 50.49 | Barclay Stephen Dale | St. Louis University | 11/4/2017 | Class 2 |
| 28 | 50.66 | Colton Smith | Parkway Central | 11/15/2024 | |
| 29 | 51.52 | Jacob Bening | Central (Cape Girardeau) | 11/3/2017 | |
| 30 | 52.38 | Jackson Garrett | Park Hill South | 11/2/2017 | Class 1 |
| 31 | 52.55 | Tyler Lewis | Lindbergh | 11/4/2017 | Class 2 |
| 32 | 52.73 | Grant Godard | Lee's Summit North | 11/4/2017 | Class 2 |
| 33 | 53.81 | Ryan Gilbert | Rock Bridge | 11/4/2017 | Class 2 |
| 34 | 54.34 | Christopher Ronald Frederick | Parkway West | 11/3/2017 | Class 1 |
| 35 | 55.50 | Alexander A Benduski | Ladue Horton Watkins | 11/3/2017 | Class 1 |
| 36 | 55.54 | Drew Benjamin Tallent | McCluer | 11/3/2017 | Class 1 |