| Rank | Time | Student | School | Date | |
| 1 | 47.46 | Cooper Jeffrey Scharff | SLUH | 11/13/2021 | |
| 2 | 47.86 | Matthew Joseph Judkins, Jr. | Chaminade College Prep | 11/11/2023 | |
| 3 | 47.90 | Jack C Ailshire | Lee's Summit West | 11/10/2022 | |
| 4 | 47.96 | Andrew Sansoucie | Timberland | 11/10/2012 | |
| 5 | 48.14 | Andrew Sansoucie | Timberland | 11/12/2011 | |
| 6 | 48.28 | Jack C Ailshire | Lee's Summit West | 11/11/2022 | |
| 7 | 48.34 | Phineas Richard Theall | Central (Cape Girardeau) | 11/15/2024 | |
| 8 | 48.38 | Caleb M Ellis | Lee's Summit West | 11/13/2021 | |
| 9 | 48.40 | Matthew Joseph Judkins, Jr. | Chaminade College Prep | 11/10/2023 | |
| 10 | 48.57 | Daniel F Worth | Lee's Summit North | 11/13/2020 | |
| 11 | 48.62 | August Alexander Brandt | Christian Brothers College | 11/9/2018 | |
| 12 | 48.76 | Matthew Joseph Judkins, Jr. | Chaminade College Prep | 11/11/2022 | Class 1 |
| 13 | 48.81 | Brogan Davis | Central (Cape Girardeau) | 11/3/2017 | Class 1 |
| 14 | 48.85 | Brogan Davis | Central (Cape Girardeau) | 11/5/2016 | |
| 15 | 48.91 | Chris McCrary | Ft. Zumwalt South | 2/11/2000 | |
| 16 | 48.94 | Nathan V Butler | Lee's Summit | 11/15/2024 | |
| 17 | 48.99 | Phineas Richard Theall | Central (Cape Girardeau) | 11/16/2024 | |
| 18 | 49.04 | Nathan V Butler | Lee's Summit | 11/14/2024 | |
| 19 | 49.20 | Nickolaus Edmond Orf | Parkway Central | 11/12/2011 | |
| 19 | 49.20 | Nickolaus Edmond Orf | Parkway Central | 11/10/2012 | |
| 21 | 49.38 | Andrew Riley Hopkins | SLUH | 11/15/2024 | |
| 22 | 49.53 | Chris McCrary | Ft. Zumwalt South | 2/12/1999 | |
| 23 | 49.54 | Jacob Schultz | Webb City | 11/7/2015 | |
| 24 | 49.59 | Max S Goldenberg | MICDS | 11/5/2016 | |
| 25 | 49.85 | Noah Samuel Witzki | Liberty | 11/4/2017 | Class 1 |
| 26 | 51.99 | Lance J Godard | Lee's Summit North | 11/4/2017 | Class 2 |
| 27 | 52.64 | Andrew William Atwood | Logan-Rogersville | 11/3/2017 | Class 1 |
| 28 | 52.70 | Mathias Jager Hostetler | St. Louis University | 11/3/1970 | Class 2 |
| 29 | 52.77 | Robert Hill | Oak Park | 11/3/2017 | Class 1 |
| 30 | 52.91 | Nick Edward Hepburn | Kirkwood | 11/3/2017 | Class 2 |
| 31 | 53.07 | Ian Berkheimer | Kickapoo | 11/3/2017 | Class 2 |
| 32 | 53.77 | Truman Vines | Central (Cape Girardeau) | 11/3/2017 | Class 1 |
| 33 | 53.81 | Drew Benjamin Tallent | McCluer | 11/3/2017 | Class 1 |