| Rank | Time | Student | School | Date | |
| 1 | 43.97 | William Jost | Parkway Central | 11/10/2023 | |
| 2 | 44.45 | Ethan Michael Vance | Camdenton | 11/10/2023 | |
| 3 | 44.56 | Liam Bresette | Lee's Summit North | 11/4/2017 | Class 2 |
| 4 | 44.73 | Matthew Ryan Margritier | Rockhurst | 11/13/2010 | |
| 5 | 44.76 | Ian Robert Everett | Liberty | 11/11/2022 | |
| 6 | 44.80 | Ian Robert Everett | Liberty | 11/10/2022 | |
| 7 | 44.93 | Sean T. McGinley | John Burroughs | 11/16/2024 | |
| 8 | 44.97 | Ian Robert Everett | Liberty | 11/11/2023 | |
| 9 | 45.04 | Caden Scott Francois | Rockhurst | 11/11/2022 | |
| 10 | 45.08 | Samuel Benjamin Senn | Kickapoo | 11/6/2015 | |
| 11 | 45.15 | Caden Scott Francois | Rockhurst | 11/10/2022 | |
| 12 | 45.24 | Per-Niklas Mikael Brinck | Nixa | 11/13/2010 | |
| 12 | 45.24 | Sean T. McGinley | John Burroughs | 11/15/2024 | |
| 14 | 45.43 | Matt Beasley | Parkway South | 2/9/2002 | |
| 15 | 45.51 | Per-Niklas Mikael Brinck | Nixa | 11/15/2008 | |
| 16 | 45.52 | Tim Patrick | Marquette | 2/10/2001 | |
| 17 | 45.53 | Matt Beasley | Parkway South | 2/10/2001 | |
| 18 | 45.55 | Albert Lloyd | Central (Springfield) | 11/15/2008 | |
| 19 | 45.58 | Matt Beasley | Parkway South | 2/10/2001 | |
| 20 | 45.64 | Brogan Davis | Central (Cape Girardeau) | 11/3/2017 | Class 1 |
| 21 | 45.77 | Cole Douglas Tobin | Liberty | 11/15/2024 | |
| 22 | 46.23 | Cole Douglas Tobin | Liberty | 11/14/2024 | |
| 23 | 46.29 | Jacob Bening | Central (Cape Girardeau) | 11/3/2017 | Class 1 |
| 24 | 46.56 | Austin Wolfe | Kearney | 11/3/2017 | Class 1 |
| 25 | 46.68 | Joseph Gerard Feder | St. Louis University | 11/4/2017 | Class 2 |
| 26 | 46.83 | Jeffrey daniel Brinck | Nixa | 11/3/2017 | Class 2 |
| 27 | 46.90 | Brooks Cosman | Christian Brothers College | 11/4/2017 | Class 2 |
| 28 | 47.37 | Benjamin Ray Hines | Nevada | 11/3/2017 | Class 1 |
| 29 | 47.51 | Ethan Kyle Bergmann | Logan-Rogersville | 11/2/1951 | Class 1 |