| Rank | AST | Student | School | Opponent | Date | |
| 1 | 19 | Becky Martin | Warren County High School | Van-Far | 12/15/1988 | |
| 1 | 19 | Danielle Markham | Advance | Caruthersville | 12/16/2020 | |
| 3 | 18 | Kenna Rae Walker | New Bloomfield | Silex | 1/26/1987 | |
| 3 | 18 | Rebecca Cook | Delta | Puxico | 2/3/2003 | |
| 5 | 17 | Rebecca Cook | Delta | Woodland | 2/13/2003 | |
| 5 | 17 | Mickayla Strother | Jamestown | Higbee | 2/2/2013 | |
| 7 | 16 | Susie Neher | Union | Bourbon | 11/15/1976 | |
| 7 | 16 | Tammy Eckart | Versailles | Osage | 1/10/1995 | |
| 7 | 16 | Tammy Eckart | Versailles | Windsor | 1/12/1995 | |
| 7 | 16 | Michelle McQueen | Lutheran South | Parkway Central | 12/28/2006 | |
| 7 | 16 | Chelsea Danielson | Crocker | Tuscumbia | 1/23/2007 | |
| 7 | 16 | Aniston Mapes | Farmington | Parkway North | 12/27/2023 | |
| 13 | 15 | Leah Cauble | Oran | | 11/24/2015 | |
| 14 | 14 | Brooklyn Shaye Luna | Fair Grove | Climax Springs | 1/9/2021 | |
| 14 | 14 | Madison Carvajal | Meadville | Tina-Avalon | 1/29/2024 | |
| 14 | 14 | Aniston Mapes | Farmington | Poplar Bluff | 2/19/2024 | |
| 14 | 14 | Aniston Mapes | Potosi | Potosi | 11/25/2024 | |
| 14 | 14 | Aniston Mapes | Potosi | Potosi | 2/27/2025 | |
| 19 | 13 | Deaven Omohundro | Clopton | Osceola | 3/1/2007 | |
| 19 | 13 | Kristin Hemken | Salem | Hillcrest | 2/23/2013 | |
| 19 | 13 | Madison Rae Ayers | South Iron | Kingston | 12/1/2021 | |
| 19 | 13 | Jersee R Wren | Southern Boone | Liberty-Christian | 2/21/2022 | |
| 19 | 13 | Aniston Mapes | Potosi | Potosi | 12/7/2024 | |
| 24 | 12 | Krista Ratliff | Salisbury | Springfield Catholic | 3/1/1989 | |
| 24 | 12 | Courtney Swift | Springfield Catholic | North Platte | 3/1/1989 | |
| 24 | 12 | Catherine Schaberg | St. Charles | | 2/20/2003 | vs. Timberland |
| 24 | 12 | Abigayle Feeney | Scotland County | | 1/16/2017 | |
| 24 | 12 | Brooklyn Shaye Luna | Fair Grove | Mountain Grove | 11/24/2020 | |
| 24 | 12 | Paidyn Linville | West Nodaway | Bishop LeBlond | 12/13/2024 | |
| 24 | 12 | Aniston Mapes | Farmington | North County | 2/28/2024 | |
| 31 | 11 | Renee Temple | Glendale | Lee's Summit | 3/1/1983 | |
| 31 | 11 | Michele Harkrader | Rich Hill | Lutheran (Kansas City) | 3/1/1988 | |
| 31 | 11 | Dawn Blaise | North Shelby | Scott County Central | 3/1/1990 | |
| 31 | 11 | Allison Murphy | Marionville | Palmyra | 3/1/1991 | |
| 31 | 11 | Erica Grimaldi | Notre Dame de Sion | Visitation Academy | 3/1/2002 | |
| 31 | 11 | Kelsi McQuinn | Jefferson (Conception) | Albany | 12/11/2015 | |
| 31 | 11 | Kelsi McQuinn | Jefferson (Conception) | West Nodaway | 2/23/2015 | |
| 31 | 11 | Brooklyn Shaye Luna | Fair Grove | Buffalo | 1/28/2020 | |
| 31 | 11 | Madison Rae Ayers | South Iron | Farmington | 2/18/2021 | |
| 31 | 11 | Madison Rae Ayers | South Iron | Bunker | 1/20/2022 | |
| 31 | 11 | Madison Rae Ayers | South Iron | Viburnum | 1/27/2022 | |
| 31 | 11 | Aniston Mapes | Farmington | Potosi | 1/25/2024 | |
| 31 | 11 | Aniston Mapes | Farmington | Dexter | 1/4/2024 | |
| 44 | 10 | Ashley Beers | Lathrop | Lafayette County | 11/28/2011 | |
| 44 | 10 | Kelsi McQuinn | Jefferson (Conception) | Northland Christian HS | 1/25/2017 | |
| 44 | 10 | Madison Rae Ayers | South Iron | Bismarck | 11/28/2020 | |
| 44 | 10 | Madison Rae Ayers | South Iron | Clearwater | 2/19/2021 | |
| 44 | 10 | Madison Rae Ayers | South Iron | Steelville | 12/28/2022 | |
| 44 | 10 | Madison Rae Ayers | South Iron | Mammoth Spring | 2/8/2023 | |
| 44 | 10 | Aniston Mapes | Farmington | Webster Groves | 12/28/2023 | |
| 44 | 10 | Korrie Holcer | Meadville | Novinger | 2/2/2024 | |
| 44 | 10 | Madison Carvajal | Meadville | Novinger | 2/2/2024 | |
| 44 | 10 | Chloe Jones | Willow Springs | Thayer | 1/10/2024 | |
| 44 | 10 | Aniston Mapes | Farmington | Ste. Genevieve | 2/6/2024 | |
| 44 | 10 | Aniston Mapes | Farmington | Arcadia Valley | 2/8/2024 | |