| Rank | Student | School | Year | | W | L |
| 1 | Emma Goodman and Sofia Smashey | Bolivar | 2024 | | 37 | 7 |
| 2 | Heather Miner/Lauren Shroyer | Lafayette (St. Joseph) | 2005 | | 34 | 3 |
| 2 | Logan Morrissey/Camryn Parnell | Lee's Summit North | 2014 | | 34 | 3 |
| 4 | Leslie Ganer/Pam Ganer | Nerinx Hall | 2006 | | 32 | 2 |
| 5 | Courtney Jahn/Lauren Owens | Forsyth | 2007 | | 31 | 2 |
| 5 | Megan Roberts and Lexi Berry | Bolivar | 2020 | | 31 | 4 |
| 7 | Logan Morrissey/Camryn Parnell | Lee's Summit North | 2013 | | 30 | 3 |
| 7 | Logan Morrissey/Camryn Parnell | Lee's Summit North | 2015 | | 30 | 3 |
| 9 | Sara Sentlinger/Deena Wallace | Ozark | 2003 | | 29 | 6 |
| 9 | Audra Feldhaus/Leslee Feldhaus | Liberty | 2006 | | 29 | 7 |
| 11 | Meghan Garlich/Megan Gorne | MICDS | 2007 | | 28 | 4 |
| 11 | Audra Feldhaus/Leslee Feldhaus | Liberty | 2007 | | 28 | 4 |
| 11 | Taylor Reese & McKenna Cox | Willow Springs | 2019 | | 28 | 4 |
| 14 | Emily Robinson/ Katie Stephens | Chillicothe | 1996 | | 27 | 0 |
| 14 | Sara Sentlinger/Deena Wallace | Ozark | 2003 | | 27 | 5 |
| 14 | Sarah Leslie/Emily Roller | Bolivar | 2000 | | 27 | 6 |
| 14 | Crystal Guiot/Julia Still | Bolivar | 2006 | | 27 | 9 |
| 14 | Crystal Guiot/Julia Still | Bolivar | 2007 | | 27 | 10 |
| 19 | Ashlee Denman/Kate Karracker | Farmington | 2001 | | 26 | 3 |
| 19 | Beth Curry/Kristyn Echterling | Bishop LeBlond | 1997 | | 26 | 5 |
| 19 | Audra Feldhaus/Maddie Morrs | Liberty | 2001 | | 26 | 5 |
| 19 | Ali Evans/Jill Spence | Liberal | 2001 | | 26 | 5 |
| 19 | Laura Owens/Kara Stauffe | Forsyth | 2006 | | 26 | 5 |
| 19 | Ali Patterson/Caroline Roth | Notre Dame de Sion | 2007 | | 26 | 7 |
| 19 | Margaret Hillin & Abigail Haley | Saxony Lutheran | 2023 | 6th in State | 26 | 7 |
| 26 | Emily Robinson/Katie Stephens | Chillicothe | 1997 | | 25 | 1 |
| 26 | Mindy Buschbom/Angela Campbell, | Savannah | 2001 | | 25 | 2 |
| 26 | Jenny Beffa/Kate Karraker | Farmington | 2003 | | 25 | 2 |
| 26 | Jessica House/Kassie McVey | Aurora | 1999 | | 25 | 3 |
| 26 | Lyndsey Hood/Kassidy Anderson | Mt. Vernon | 2018 | | 25 | 5 |
| 26 | McKylie Cox & Madisyn Perkins | Willow Springs | 2022 | | 25 | 5 |
| 26 | Courtney Jahn/Jordan Schanda | Forsyth | 2006 | | 25 | 6 |
| 26 | Ashley O’Dell/Jennifer Stricker, | Glendale | 2005 | | 25 | 9 |
| 34 | Abigail Haley & Margaret Hillin | Saxony Lutheran | 2024 | | 24 | 3 |
| 35 | Whitney Wiehe/Abby Rinker Doubles | Mt. Vernon | 2015 | 8th in State | 23 | 2 |
| 36 | Erin Jarvis/Casey Lee | Springfield Catholic | 2018 | | 22 | 1 |
| 36 | Taylor Reese & McKenna Cox | Willow Springs | 2018 | | 22 | 7 |
| 38 | Whitney Wiehe/Kaylee Hood Doubles | Mt. Vernon | 2014 | 16th in State | 21 | 1 |
| 38 | Lyndsey Hood/Stevie Meirick Double | Mt. Vernon | 2017 | 16th in State | 21 | 3 |
| 40 | Abby Rinker/Alane Cordray | Mt. Vernon | 2017 | | 20 | 1 |
| 40 | Taylor Reese & McKenna Cox | Willow Springs | 2020 | | 20 | 3 |
| 40 | Margaret Hillin & Abigail Haley | Saxony Lutheran | 2022 | 7th in State | 20 | 11 |