| Rank | ACE | Student | School | Opponent | Date | |
| 1 | 27 | Emerson Walter | Platte County | Platte County | 9/24/2020 | |
| 2 | 20 | Lexi Ferguson | Mid-Buchanan | Hogan Prep Academy Charter | 9/28/2021 | |
| 3 | 17 | Amber Rutherford | West Platte | Hogan Prep Academy Charter | 10/21/2025 | |
| 4 | 16 | Paige Gordon | Drexel | Kingsville | 9/21/2021 | |
| 5 | 15 | Sara Gregor | Hazelwood West | Hazelwood East | 9/21/2011 | |
| 6 | 14 | Kaitlyn Belk | Bunker | Couch | 10/1/2021 | |
| 7 | 13 | Anna Vogt | Pacific | Crystal City | 8/31/2011 | |
| 7 | 13 | Abigail Cole | Licking | Dixon | 9/25/2017 | |
| 9 | 12 | Chrissy Elder | Park Hill South | Center | 10/8/1998 | |
| 9 | 12 | Kelly Desmond | Thomas Jefferson Independent | Sarcoxie | 9/1/1998 | |
| 9 | 12 | Morgyn Bergschneider | Archie | Sherwood | 9/6/2005 | |
| 9 | 12 | Lydia Klenke | Union | St. James | 10/1/2015 | |
| 9 | 12 | Taylor Osborne | Winona | Couch | 9/28/2021 | |
| 14 | 11 | Rose Swartz | East Newton | Bentonville, Ark. | 8/31/2000 | |
| 14 | 11 | Tori Jenea Naylor | Cameron | Warsaw | 9/10/2011 | |
| 14 | 11 | Analee Tucker | Marshall | Richmond | 10/28/2013 | |
| 14 | 11 | Karma Romine | Southwest (Washburn) | | 10/18/2019 | vs. Exeter |
| 14 | 11 | Tisha Mackey | Rich Hill | Hume | 10/28/2020 | |
| 14 | 11 | Lia Graham | Plattsburg | Lathrop | 9/30/2021 | |
| 14 | 11 | Audrey Marie Trout | Lawson | University Academy Charter | 10/24/2022 | |
| 21 | 10 | Lisa Downey | Lafayette (Wildwood) | Lindbergh | 8/29/2000 | |
| 21 | 10 | Zora Whitt | East Buchanan | Penney | 9/30/2021 | |
| 21 | 10 | Callie Sutton | Bunker | Marquand-Zion | 9/2/2021 | |
| 21 | 10 | Libby Juergensmeyer | Blair Oaks | Couch | 9/10/2022 | |
| 25 | 9 | Lily Johnson | Lafayette (Wildwood) | Westminster Christian Academy | 10/1/2013 | |
| 25 | 9 | Zora Whitt | East Buchanan | Plattsburg | 8/28/2021 | |
| 25 | 9 | Catherine Elizabeth Woolf | North Point | Winfield | 9/15/2022 | |
| 25 | 9 | Heidi Roweton | Bolivar | Parkview | 10/16/2023 | |
| 29 | 8 | Renee Westmoreland | Clearwater | Unknown | 11/1/1988 | Class 2 |
| 29 | 8 | Serena J Sundell | Maryville | Lafayette (St. Joseph) | 9/17/2020 | |
| 29 | 8 | Kailey Belk | Bunker | Couch | 10/1/2021 | |
| 29 | 8 | Kylee Quick | Bunker | Viburnum | 10/14/2021 | |
| 29 | 8 | Loren Ritter | Bunker | Dora | 9/15/2022 | |
| 29 | 8 | Jadyn Paige Howard | Carl Junction | Joplin | 10/14/2024 | at Joplin HS |
| 29 | 8 | Ziah Schmutz | Mt. Vernon | East Newton | 10/1/2024 | 3 sets played |
| 36 | 7 | Kaleigh Rain Cole | Nixa | Logan-Rogersville | 10/18/2012 | |
| 36 | 7 | Mckenzie G Hines | Nixa | Carthage | 9/26/2019 | |
| 36 | 7 | Audrey Kathryn Small | Ft. Zumwalt West | Jacksonville, IL | 10/8/2022 | |
| 36 | 7 | Emma Elaine Conrad | Nixa | Neosho | 9/20/2022 | |
| 36 | 7 | Camryn Cornell | Mt. Vernon | Seneca | 10/15/2024 | 3 sets played |
| 36 | 7 | Macie West | Mt. Vernon | McDonald County | 10/3/2024 | 3 sets played |
| 36 | 7 | Ziah Schmutz | Mt. Vernon | Saxony Lutheran | 11/7/2025 | Class 3 |
| 43 | 6 | Makayla Stephen | Nixa | Neosho | 10/15/2013 | |
| 43 | 6 | Rielly Dobbs | Nixa | Neosho | 10/29/2013 | |
| 43 | 6 | Aubrey Cheffey | Nixa | Parkview | 10/20/2014 | |
| 43 | 6 | Mckenzie G Hines | Nixa | Lee's Summit West | 9/29/2018 | |
| 43 | 6 | Taylor Golmen | Nixa | Branson | 10/6/2020 | |
| 43 | 6 | Jena Lee Medearis | Nixa | Cor Jesu Academy | 9/25/2021 | |
| 49 | 5 | Kelly Shearer | Bernie | Unknown | 11/1/1994 | Class 1 |
| 49 | 5 | Courtney Curtiss | Sacred Heart | Unknown | 11/1/2008 | Class 1 |
| 49 | 5 | Kelsey D Scott | Maryville | East Buchanan | 9/3/2020 | |
| 49 | 5 | Morgan L Stoecklein | Maryville | Southwest Valley | 9/8/2020 | |
| 49 | 5 | Lauren Elizabeth Sloniker | Nevada | Summit Christian Academy | 9/11/2025 | |
| 49 | 5 | Halle Jo Hawks | Nevada | Monett | 10/9/2025 | |
| 49 | 5 | Averee Swillum | Mt. Vernon | Helias Catholic | 11/8/2025 | Class 3 |
| 49 | 5 | Lexi Lindsey | Winona | Northland Christian | 11/7/2025 | Class 1 |
| 57 | 4 | Michelle Oldani | Bishop DuBourg | Unknown | 11/1/1997 | Class 3 |
| 57 | 4 | Katie Duchek | Cor Jesu Academy | Unknown | 11/1/2001 | Class 4 |
| 57 | 4 | Claire Ulrich | Lafayette (Wildwood) | Lee's Summit North | 11/7/2025 | Class 5 |
| 57 | 4 | Piper Hudson | Oak Grove | Saxony Lutheran | 11/8/2025 | Class 3 |
| 57 | 4 | Ashley Hawkins | Bismarck | Liberal | 11/7/2025 | Class 1 |
| 57 | 4 | Savannah Dawn Crane | Webb City | Hannibal | 11/6/2025 | Class 4 |
| 57 | 4 | Audrey Schaeffer | Incarnate Word Academy | Lee's Summit North | 11/6/2025 | Class 5 |
| 57 | 4 | Audrey Schaeffer | Incarnate Word Academy | Nixa | 11/7/2025 | Class 5 |
| 57 | 4 | Kate Sherry | St. Pius X (Festus) | Webb City | 11/5/2025 | Class 4 |