| Rank | Student | Grade | School | Year | | W | L |
| 1 | Kelsey Kessler | Fr | Blue Springs | 2008 | | 31 | 0 |
| 2 | Kennedy Lee Diggs | Jr | Sherwood | 2022 | | 30 | 6 |
| 3 | Harley Daniels | Sr | Mt. Vernon | 2022 | | 29 | 1 |
| 3 | Melanie Wallach | Sr | Eureka | 1999 | | 29 | 3 |
| 5 | Megan Daniels | Sr | Liberty | 2004 | | 28 | 1 |
| 5 | Lauren Reinagel | So | Notre Dame (Cape Girardeau) | 2007 | | 28 | 1 |
| 5 | Sophia Alexander | Jr | Strafford | 2005 | | 28 | 2 |
| 5 | Lauren Reinagel | Sr | Notre Dame (Cape Girardeau) | 2009 | | 28 | 2 |
| 9 | Lauren Reinagel | Jr | Notre Dame (Cape Girardeau) | 2008 | | 27 | 1 |
| 9 | Sarah Siegismund | Jr | Pleasant Hope | 2011 | | 27 | 1 |
| 11 | Kenya Fuemmeler | Sr | Salisbury | 2002 | | 26 | 0 |
| 11 | Madison McKinney | Sr | Norborne | 2015 | | 26 | 0 |
| 11 | Taylor Baxter | Fr | Palmyra | 2006 | | 26 | 1 |
| 11 | Brooke Huitt | Jr | Lathrop | 2017 | | 26 | 1 |
| 11 | Mac Morgan | Jr | Sherwood | 2019 | | 26 | 3 |
| 11 | Andrea Studer | So | Reeds Spring | 2010 | | 26 | 4 |
| 11 | Brittany Nimmo | Sr | Ozark | 2014 | | 26 | 4 |
| 11 | Kinlei Boley | Jr | Chillicothe | 2021 | | 26 | 4 |
| 11 | Hailey Presley | Jr | Marion C. Early | 2022 | | 26 | 8 |
| 20 | Brooke Huitt | So | Lathrop | 2016 | | 25 | 3 |
| 21 | Jaylynn J Brown | So | Centralia | 2024 | | 24 | 2 |
| 21 | Abigail Lay | Jr | Highland | 2024 | | 24 | 5 |
| 23 | Tressa Hughes | Sr | Dixon | 2017 | | 23 | 1 |
| 23 | Ainsley Tolson | Sr | Trenton | 2018 | | 23 | 2 |
| 23 | Jaydin Lee | Jr | Pleasant Hope | 2018 | | 23 | 4 |
| 26 | Taylor LeeAnn Baumhoer | Sr | Fatima | 2023 | | 22 | 1 |
| 26 | Hannah Carter | Sr | South Harrison | 2023 | | 22 | 6 |
| 26 | Hailey Presley | Sr | Marion C. Early | 2023 | | 22 | 12 |
| 26 | Grace Osborne | Jr | Jasper | 2022 | | 22 | 13 |
| 30 | Hannah Carter | Jr | South Harrison | 2022 | | 21 | 5 |
| 31 | Taylor Brown | Jr | Washington | 2022 | | 20 | 2 |