| Rank | AST | School | Opponent | Date | |
| 1 | 49 | Rosary | Central Visual and Performing Arts | 2/24/1998 | at Soldan |
| 2 | 47 | Chadwick | Lutie | 12/15/2011 | |
| 3 | 36 | New Bloomfield | Osage | 11/30/1987 | |
| 3 | 36 | Warren County | Lutheran St. Charles | 1/16/1994 | |
| 5 | 34 | Clopton | Louisiana | 1/23/2006 | |
| 6 | 33 | Putnam County | Mercer | 1/12/1998 | |
| 7 | 32 | St. James | Arcadia Valley | 12/26/2001 | |
| 7 | 32 | Chadwick | Niangua | 1/8/2002 | |
| 7 | 32 | Chadwick | Galena | 2/15/2002 | |
| 7 | 32 | Skyline | Richmond | 12/20/2002 | |
| 7 | 32 | Stanberry | Tarkio | 2/19/2002 | |
| 12 | 30 | Meadville | Bevier | 1/10/2023 | 30 assists |
| 12 | 30 | Meadville | Northwestern (Mendon) | 11/30/2023 | |
| 12 | 30 | Meadville | Novinger | 2/2/2024 | |
| 15 | 28 | Southern Boone | Community | 1/30/1987 | |
| 15 | 28 | Twin Rivers | Caruthersville | 1/26/2021 | |
| 15 | 28 | Meadville | Tina-Avalon | 1/29/2024 | |
| 18 | 26 | Meadville | Tina-Avalon | 1/30/2023 | 26 assists |
| 18 | 26 | Tipton | Eugene | 1/21/2025 | |
| 20 | 25 | Meadville | La Plata | 1/13/2023 | 25 assists |
| 20 | 25 | Meadville | Northwestern (Mendon) | 1/20/2023 | 25 assists |
| 20 | 25 | Meadville | Winston | 12/14/2023 | |
| 20 | 25 | Meadville | Bucklin | 1/23/2024 | |
| 20 | 25 | Tipton | St. James | 1/15/2025 | |
| 25 | 24 | Scott County Central | Gainesville | 3/1/1982 | |
| 25 | 24 | Scott County Central | Gainesville | 3/1/1982 | |
| 25 | 24 | Meadville | Glasgow | 11/17/2023 | |
| 25 | 24 | Meadville | Brashear | 12/8/2023 | |
| 25 | 24 | Lathrop | Braymer | 1/21/2025 | |
| 30 | 23 | Springfield Catholic | North Platte | 3/1/1989 | |
| 30 | 23 | Springfield Catholic | North Platte | 3/1/1989 | |
| 30 | 23 | Grundy County | Winston | 12/17/2019 | |
| 30 | 23 | Meadville | Green City | 1/17/2023 | 23 assists |
| 30 | 23 | Meadville | Linn County | 12/12/2023 | |
| 30 | 23 | Meadville | Hale | 2/1/2024 | |
| 30 | 23 | Meadville | Grundy County | 2/15/2024 | |
| 30 | 23 | Meadville | Northwestern (Mendon) | 2/21/2024 | |
| 38 | 22 | Marionville | Eskridge | 3/1/1991 | |
| 38 | 22 | Marionville | Eskridge | 3/1/1991 | |
| 38 | 22 | St. Joseph's Academy | Kickapoo | 3/1/1994 | |
| 38 | 22 | St. Joseph's Academy | Kickapoo | 3/1/1994 | |
| 38 | 22 | Meadville | Bucklin | 2/10/2023 | 22 assists |
| 38 | 22 | Meadville | Brunswick | 2/23/2024 | |
| 38 | 22 | Meadville | Green City | 2/26/2024 | |
| 45 | 21 | Rich Hill | Lutheran (Kansas City) | 3/1/1988 | |
| 45 | 21 | Rich Hill | Lutheran (Kansas City) | 3/1/1988 | |
| 45 | 21 | Salisbury | Springfield Catholic | 3/1/1989 | |
| 45 | 21 | Salisbury | Springfield Catholic | 3/1/1989 | |
| 45 | 21 | Meadville | Scott County Central | 3/1/1995 | |
| 45 | 21 | Meadville | Scott County Central | 3/1/1995 | |
| 45 | 21 | Lathrop | Lexington | 11/22/2022 | |
| 45 | 21 | Lathrop | Penney | 2/13/2024 | |
| 45 | 21 | Meadville | Bevier | 1/31/2024 | |
| 45 | 21 | Meadville | Green City | 2/12/2024 | |
| 55 | 20 | Lathrop | Polo | 1/13/2011 | |
| 55 | 20 | Lathrop | Plattsburg | 12/15/2022 | |
| 55 | 20 | Lathrop | KIPP KC Legacy | 11/30/2023 | |
| 55 | 20 | Meadville | Bishop LeBlond | 2/5/2024 | |
| 55 | 20 | Meadville | La Plata | 2/7/2024 | |
| 55 | 20 | Lathrop | Penney | 2/20/2025 | |