| Rank | RB | Student | School | Opponent | Date | |
| 1 | 38 | Beth Dennis | Higbee | Bunceton | 2/17/1983 | |
| 2 | 37 | Ann Monachino | St. Pius X (Kansas City) | Bishop Hogan | 2/5/1979 | |
| 3 | 36 | Antionette Wells | Northeast (Kansas City) | Central (Kansas City) | 2/8/2002 | |
| 4 | 33 | Denise Toney | Kearney | Mid-Buchanan | 11/16/1972 | |
| 4 | 33 | Sherri Sumpter | Higbee | Bunceton | 2/17/1983 | |
| 6 | 32 | Mary Brueggestras | St. Joseph's Academy | Visitation Academy | 12/31/1979 | |
| 6 | 32 | Kelly Baldon | Polo | Concordia | 2/6/2020 | |
| 8 | 31 | Stacey Fritts | Miami | Metz | 12/16/1983 | |
| 8 | 31 | Kesha Bonds | Winnetonka | Excelsior Springs | 2/2/1995 | |
| 10 | 30 | Kari Koch | Elsberry | Southern Boone | 3/10/2001 | |
| 10 | 30 | Nikki Knapp | Blue Springs South | Lee's Summit | 1/3/2002 | |
| 10 | 30 | Lauren Dubbert | Madison | Brunswick | 1/16/2004 | |
| 13 | 28 | Jandie Peterson | Grundy County | Tri-County | 2/15/2020 | |
| 14 | 27 | Paige Tate | Spokane | Purdy | 2/10/2020 | |
| 14 | 27 | Mackinley Aaron | Cabool | Norwood | 2/19/2022 | |
| 14 | 27 | Jaryn Hatcher | Green City | Brashear | 1/28/2025 | |
| 17 | 26 | Morgan Johnson | Platte County | Webb City | 3/1/2009 | |
| 17 | 26 | Maggie Collins | Jefferson (Conception) | South Harrison | 12/6/2022 | |
| 17 | 26 | Tayja Marie Carter | Kennett | East Carter | 2/13/2024 | |
| 20 | 25 | Isabel Maddick | Lathrop | Polo | 2/21/2012 | |
| 20 | 25 | Reese Gray | Lesterville | St. Paul Lutheran (Farmington) | 12/5/2020 | |
| 20 | 25 | Briar Cox | Tipton | Capital City | 12/4/2021 | |
| 20 | 25 | Brenna Couch | Plato | Summersville | 2/13/2023 | |
| 20 | 25 | Lillian Thompson | Marion C. Early | Blue Eye | 12/23/2024 | |
| 25 | 24 | Paige Tate | Spokane | Galena | 1/21/2019 | |
| 25 | 24 | Kylee Richey | Versailles | Smithton | 12/7/2021 | |
| 25 | 24 | Nyrie Allen | Northeast (Kansas City) HS | Central (Kansas City) HS | 2/1/2022 | |
| 25 | 24 | Lauren Grace Bartlett | Osage | Eldon | 2/28/2024 | |
| 29 | 23 | Stacy Nunn | Marshfield | Duchesne | 3/1/1988 | |
| 29 | 23 | Alicia Rae Hager | Lutheran South | | 12/2/2010 | |
| 29 | 23 | Tori Meinecke | Mercer | Princeton | 1/18/2020 | |
| 29 | 23 | Paige Tate | Spokane | Clever | 1/9/2020 | |
| 29 | 23 | Paige Tate | Spokane | Galena | 1/27/2020 | |
| 29 | 23 | Ashton Bailey Judd | West Plains | Nixa | 12/31/2020 | |
| 29 | 23 | Brenna Couch | Plato | Stoutland | 12/19/2023 | |
| 29 | 23 | Ava Graham | West Nodaway | Westran | 12/14/2024 | |
| 29 | 23 | Ava Schlotzhauer | Tipton | Helias Catholic | 12/29/2024 | |
| 38 | 22 | Gabrielle L. Walker | St. Charles | | 1/30/2015 | vs Ladue |
| 38 | 22 | Gabrielle L. Walker | St. Charles | | 2/24/2015 | |
| 38 | 22 | Leketor Member-Meneh | Lutheran South | | 2/2/2017 | |
| 38 | 22 | Paige Tate | Spokane | Verona | 11/22/2019 | |
| 38 | 22 | Paige Tate | Spokane | Parkview | 12/27/2019 | |
| 38 | 22 | Juliann Smith | Bishop LeBlond | Bishop LeBlond | 2/24/2020 | |
| 38 | 22 | Paige Tate | Spokane | New Covenant Academy | 1/16/2020 | |
| 38 | 22 | Kaylee Missa Hilton | Hillsboro | Cuba | 12/29/2021 | |
| 38 | 22 | Brenna Couch | Plato | Central (Springfield) | 1/20/2024 | |
| 47 | 21 | Nancy Brandt | Fairfax | Northwestern (Mendon) | 3/1/1980 | |
| 47 | 21 | Marian Fitzsimmons | Macon County | Maryville | 3/1/1981 | |
| 47 | 21 | Virginia Blissett | Scott County Central | Salisbury | 3/1/1982 | |
| 47 | 21 | Tonya Harry | Brentwood | Bishop LeBlond | 3/1/1986 | |
| 47 | 21 | Shatonya Fort | Scott County Central | Wellington-Napoleon | 3/1/1993 | |
| 47 | 21 | Jesse Fletcher | Atlanta | Greenwood | 3/1/1997 | |
| 47 | 21 | Kaci Rae Sargent | Meadville | Macks Creek | 3/1/2008 | |
| 47 | 21 | Gabrielle L. Walker | St. Charles | | 12/2/2015 | vs. Holt |
| 47 | 21 | Aubrie McRoberts | Warsaw | Pilot Grove | 12/28/2016 | |
| 47 | 21 | Hayley Frank | Strafford | Lutheran North | 3/1/2017 | |
| 47 | 21 | Milliana Chyane Mozee-Williams | South Shelby | Canton | 12/15/2020 | |
| 47 | 21 | Stemaiya Robinson | Hayti | Campbell | 1/21/2021 | |
| 47 | 21 | Brenna Couch | Plato | Bradleyville | 2/10/2023 | |
| 47 | 21 | Khylie Remmenga | Knob Noster | Boonville | 2/28/2023 | |
| 47 | 21 | Enzley Dinkins | South Iron | Lesterville | 11/30/2023 | |
| 62 | 20 | Shianne Rhorer | Prairie Home | | 11/22/2016 | |
| 62 | 20 | Kamryn Elise-dawn Gastineau | Liberty (Mountain View) | | 2/15/2018 | vs. Cabool |
| 62 | 20 | Kaitlyn King | Liberty (Mountain View) | | 2/18/2019 | |
| 62 | 20 | Jaycee D Callaway | Niangua | Fordland | 1/10/2020 | |
| 66 | 19 | Eden Grace Robart | Potosi | Potosi | 1/13/2025 | |