| Rank | Student | School | Years | | W | L |
| 1 | Kaylee Davis | Doniphan | 2015-2018 | | 99 | 48 |
| 2 | Kelsey Kessler | Blue Springs | 2008-2011 | | 92 | 16 |
| 3 | Taylor Baxter | Palmyra | 2006-2009 | | 88 | 5 |
| 4 | Lauren Delaney | Helias Catholic | 2002-2005 | | 84 | 3 |
| 4 | Lauren Reinagel | Notre Dame (Cape Girardeau) | 2006-2009 | | 84 | 4 |
| 6 | Brooke Huitt | Lathrop | 2015-2018 | | 83 | 9 |
| 6 | Valerie Teter | Bevier | 2003-2006 | | 83 | 13 |
| 6 | Sarah Baldwin | Chillicothe | 2008-2011 | | 83 | 17 |
| 9 | Cola Krueger | Blue Springs South | 2001-2004 | | 81 | 26 |
| 10 | Traci Mothersbaugh | Putnam County | 1988-1991 | | 80 | 9 |
| 10 | Dana Halstead | La Plata | 1998-2001 | | 80 | 17 |
| 12 | Megan Summers | Westran | 2003-2006 | | 77 | 8 |
| 13 | Meghan Bailey | Sullivan | 2002-2005 | | 76 | 28 |
| 13 | Hannah Harman | Walnut Grove | 2007-2010 | | 76 | 28 |
| 15 | Brianna Isabelle Lore | Sullivan | 2008-2011 | | 75 | 21 |
| 16 | Tressa Hughes | Dixon | 2014-2017 | | 71 | 26 |
| 17 | Ainsley Tolson | Trenton | 2016-2019 | | 66 | 8 |