| Rank | AST | Student | School | Opponent | Date | |
| 1 | 50 | | Notre Dame (Cape Girardeau) | N/A | 1/1/1987 | Class 2A |
| 2 | 44 | | Lafayette (St. Joseph) | N/A | 1/1/1975 | Class 3A |
| 2 | 44 | | Hartville | N/A | 1/1/1998 | Class 2A |
| 4 | 41 | | Clayton | N/A | 1/1/1975 | Class 3A |
| 5 | 40 | | McCluer | N/A | 1/1/1975 | Class 4A |
| 5 | 40 | | Raytown South | N/A | 1/1/1975 | Class 4A |
| 5 | 40 | | Scott County Central | N/A | 1/1/1987 | Class 1A |