| Rank | Time | Student | School | Date | |
| 1 | 52.72 | Isabelle Cassidy Ackley | Timberland | 2/16/2024 | |
| 2 | 53.78 | Isabelle Cassidy Ackley | Timberland | 2/17/2024 | |
| 3 | 54.09 | Taylor Wohrley | Timberland | 2/20/2009 | |
| 4 | 54.37 | Haley Marie Hynes | Lee's Summit North | 2/22/2014 | |
| 5 | 54.39 | Molly Moore | Summit Prep | 2/16/2018 | Class 1 |
| 6 | 54.46 | Isabelle Cassidy Ackley | Timberland | 2/17/2023 | |
| 7 | 54.60 | Sydney Schoeck | Westminster Christian Academy | 2/17/2023 | |
| 8 | 54.63 | Avery Allison Cunneen | St. Joseph's Academy | 2/21/2025 | |
| 9 | 54.68 | Isabelle Cassidy Ackley | Timberland | 2/16/2023 | |
| 10 | 54.93 | Avery Allison Cunneen | St. Joseph's Academy | 2/22/2025 | |
| 11 | 54.98 | Gabriela M. Vieira | Parkway West | 2/20/2016 | |
| 12 | 55.06 | Sydney Schoeck | Westminster Christian Academy | 2/18/2023 | |
| 13 | 55.11 | Haley Marie Hynes | Lee's Summit North | 2/16/2013 | |
| 14 | 55.15 | Rebekah Land | Hannibal | 2/19/2011 | |
| 15 | 55.49 | Madison Janelle Brown | Parkway Central | 2/18/2017 | |
| 16 | 55.81 | Lauren Shaw | Park Hill | 2/16/2013 | |
| 17 | 56.04 | Caroline Rodriguez | Lafayette (Wildwood) | 5/22/2004 | |
| 17 | 56.04 | Audrey Schank | Central (St. Joseph) | 2/17/2017 | |
| 19 | 56.27 | Elizabeth Brianna Landuyt | Park Hill South | 2/21/2025 | |
| 20 | 56.49 | Karisa Franz | Cor Jesu Academy | 2/17/2018 | Class 2 |
| 21 | 56.90 | Elizabeth Brianna Landuyt | Park Hill South | 2/20/2025 | |
| 22 | 56.93 | Georgia Clark | Park Hill South | 2/17/2018 | Class 2 |
| 23 | 57.27 | Ellie Zweifel | Rock Bridge | 2/17/2018 | Class 2 |
| 24 | 57.38 | Ella Grace Pearl | Kirkwood | 2/17/2018 | Class 2 |
| 25 | 57.74 | Olivia Belle Henderson | Rock Bridge | 2/16/2018 | Class 2 |
| 26 | 59.01 | Parker E Hagemann | Webster Groves | 2/15/2018 | Class 1 |
| 27 | 59.78 | Caroline Martucci | Thomas Jefferson Independent | 2/16/2018 | Class 1 |
| 28 | 59.85 | Madeleine Duncan | Glendale | 2/16/2018 | Class 1 |
| 29 | 1:00.44 | Ellie Hartwig | St. Pius X (Kansas City) | 2/16/2018 | Class 1 |