| Rank | Time | Student | School | Date | |
| 1 | 53.53 | Daniel F Worth | Lee's Summit North | 11/16/2019 | |
| 2 | 53.81 | Daniel F Worth | Lee's Summit North | 11/15/2019 | |
| 3 | 54.10 | Trey Matthew Clervi | Rock Bridge | 11/14/2024 | |
| 4 | 54.15 | Daniel F Worth | Lee's Summit North | 11/13/2020 | |
| 5 | 54.21 | Trey Matthew Clervi | Rock Bridge | 11/15/2024 | |
| 6 | 54.27 | William Austin Bonnett | Parkway West | 11/12/2021 | |
| 7 | 54.53 | William Austin Bonnett | Parkway West | 11/12/2020 | |
| 8 | 54.78 | Jeff Commings | St. Louis University | 2/23/1991 | |
| 9 | 54.86 | Connor Muran | Parkway Central | 11/14/2025 | |
| 10 | 55.00 | Ryan James Coughenour | Rock Bridge | 11/15/2025 | |
| 11 | 55.09 | Alexander Crawford | Joplin | 11/16/2019 | |
| 12 | 55.12 | Daniel F Worth | Lee's Summit North | 11/16/2018 | |
| 13 | 55.31 | Noah M Reeves | Lee's Summit | 11/14/2024 | |
| 14 | 55.55 | Jeff Commings | St. Louis University | 2/24/1990 | |
| 15 | 55.61 | Noah M Reeves | Lee's Summit | 11/15/2024 | |
| 16 | 55.71 | Atticus Sternke | Hannibal | 11/16/2024 | |
| 17 | 55.80 | Atticus Sternke | Hannibal | 11/15/2024 | |
| 18 | 55.85 | Connor Muran | Parkway Central | 11/15/2024 | |
| 19 | 55.96 | Trevor Hansen | Parkway Central | 11/12/2022 | |
| 20 | 56.03 | Sam Drew | Lee's Summit | 11/5/2016 | |
| 21 | 56.05 | Mason Weber | Marshall | 11/15/2008 | |
| 22 | 56.11 | Nathan Wu | Greenwood | 11/16/2024 | |
| 23 | 56.41 | Andrew Simek | Ft. Zumwalt West | 11/15/2008 | |
| 24 | 56.87 | Elliott L Eisele-Miller | Oak Park | 11/7/2015 | |
| 25 | 56.88 | Sam Drew | Lee's Summit | 11/7/2015 | |
| 26 | 56.93 | Connor T Bichsel | Lee's Summit West | 11/13/2021 | |
| 27 | 56.95 | Grant Sloan | Kearney | 11/3/2017 | Class 1 |
| 28 | 57.01 | Tyler Nichols | Marshall | 11/12/2011 | |
| 29 | 57.02 | Anthony Alexander Albracht | Rockhurst | 11/8/2014 | |
| 30 | 57.07 | Daniel Worth | Lee's Summit North | 11/4/2017 | Class 2 |
| 31 | 57.10 | Andy Huffman | Lee's Summit | 11/4/2017 | Class 2 |
| 32 | 57.39 | Sam Hahs | Central (Cape Girardeau) | 11/3/2017 | Class 1 |
| 33 | 57.69 | Zachary Christian Lorson | Rock Bridge | 11/3/2017 | Class 2 |
| 34 | 58.69 | Joseph Austin Lemon | Marquette | 11/4/2017 | Class 2 |
| 35 | 58.89 | Joseph Trout | Liberty North | 11/4/2017 | Class 2 |
| 36 | 59.11 | Henry Feyh | Glendale | 11/3/2017 | Class 1 |
| 37 | 59.18 | Matthew States | Lutheran St. Charles | 11/3/2017 | Class 1 |
| 38 | 1:00.59 | Caden DeLay | Platte County | 11/2/2017 | Class 1 |